摘要：Assembling protective metal-organic framework (MOF) coatings for structurally fragile natural enzymes broadens their potential industrial applications. In this study, we developed an enhanced two-step biomimetic mineralization strategy, whereby cellulase acts as a biological template to pre-concentrate Zn2+ on its surface, subsequently initiating the rapid in situ formation of zeolitic imidazolate framework-8 (ZIF-8). This approach yields a cellulase@ZIF-8 composite that effectively combines enzyme activity retention with the structural robustness of the MOF. Cellulase actively mediated ZIF-8 nucleation and framework construction through precise interfacial coordination with imidazole ligands and Zn2+, thus achieving in situ encapsulation. Compared with free cellulase, cellulase@ZIF-8 maintained over 90 % residual activity under acidic conditions and over 60 % activity at 80 °C. Remarkably, under alkaline conditions (pH = 9) or after 30 days of storage, cellulase@ZIF-8 residual activity exceeded twice that of the free enzyme. Even after 10 recycles, cellulase@ZIF-8 retained 60.5 % residual activity, exhibiting outstanding environmental tolerance and reusability. Our results suggest that the spatial confinement provided by ZIF-8, along with its ability to induce conformational rearrangement in the enzyme, underlies the enhanced stability and environmental tolerance observed. We further showed a pH-responsive release of cellulase facilitated by the ZIF-8 framework, enabling burst release of ＞90 % cellulase in a short time under acidic environment. This work offers valuable insights into enzyme-MOFs design and their applications in biocatalysis, controlled release, and adaptive biomaterials. ? 2025 Elsevier B.V.

ISSN号：1385-8947

卷、期、页：卷522,

发表日期：2025-10-15

影响因子：0.000000

期刊分区（SCI为中科院分区）：一区

收录情况：SCI（科学引文索引印刷版）,EI（工程索引）,SCIE（科学引文索引网络版）

发表期刊名称：Chemical Engineering Journal

参与作者：Husein, Maen M.,康傅艳

通讯作者：杨俊,张伟,黄胜铭

第一作者：蒋官澄,董腾飞,贺垠博,杨丽丽

论文类型：期刊论文

论文概要：杨俊,蒋官澄,Husein, Maen M.,张伟,黄胜铭,康傅艳,董腾飞,贺垠博,杨丽丽，Biomimetic mineralization-driven in situ encapsulation of cellulase into ZIF-8: Enhanced enzyme stability and pH-responsive release，Chemical Engineering Journal，2025，卷522,

论文题目：Biomimetic mineralization-driven in situ encapsulation of cellulase into ZIF-8: Enhanced enzyme stability and pH-responsive release