摘要：DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstream molecular biology research. In this study, three different spectrophotometry methods, BPM, NDTT and NPMTTZ were compared for their performance in determining DNA concentration and purity in 32 oil samples, and molecule methods like quantitative real-time PCR (qPCR) and high-throughput sequence were also performed to help assess the accuracy of the three methods in determining DNA concentration and purity. For ordinary heavy oil (OHO), extra heavy oil (EHO) and super heavy oil (SHO), the characteristics of high viscosity (η), density (ρ) and resin plus asphaltene content will affect the DNA extraction and UV determination. The DNA concentration was decreased as density increased: OHO (11.46 ± 18.34 ng/μL), EHO (6.68 ± 9.67 ng/μL) and SHO (6.20 ± 7.83 ng/μL), and the DNA purity was on the reverse: OHO (1.31 ± 0.27), EHO (1.54 ± 0.20), and SHO (1.83 ± 0.32). The results suggest that spectrophotometry such as BPM and NPMTTZ are qualitatively favorite methods as the quick non-consumable methods in determining DNA concentration and purity of medium oil and heavy oil. ? 2023 The Authors

ISSN号：1672-5107

卷、期、页：卷20期6：3394-3399

发表日期：2023-12-01

影响因子：0.000000

期刊分区（SCI为中科院分区）：二区

收录情况：SCI（科学引文索引印刷版）,CSCD（中国科技引文期刊）（核心）,EI（工程索引）,SCIE（科学引文索引网络版）

发表期刊名称：Petroleum Science

参与作者：Yang, JianPing,Dong, HaiLiang

通讯作者：穆红梅,罗娜

第一作者：万云洋

论文类型：期刊论文

论文概要：万云洋,穆红梅,罗娜,Yang, JianPing,Dong, HaiLiang，Rapid accuracy determining DNA purity and concentration in heavy oils by spectrophotometry methods，Petroleum Science，2023，卷20期6：3394-3399

论文题目：Rapid accuracy determining DNA purity and concentration in heavy oils by spectrophotometry methods