摘要：Biodegradation preference of single, identified bacterium is the basis of bioremediation and microbial enhanced oil recovery (MEOR). However, the impacts of different bacterial species on the biodegradation of polar compounds are unclear. Accordingly, in this study, we performed a 90-day biodegradation simulation of crude oil using Pseudomonas aeruginosa XJ16 and Acinetobacter lwoffii XJ19. Biodegraded oils were characterized by negative-ion electrospray source ionization ESI (-), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), and gas chromatography-mass spectrometry (GC-MS). We aimed to study the differential biodegradation and mechanism of nitrogen- and oxygen-containing polar compounds using two strains that showed different abilities to produce and utilize polar compounds. P. aeruginosa XJ16 could easily biodegrade n-alkanes and n-alkyl cyclohexanes, whereas A. lwoffii XJ19 was able to utilize complex compounds, such as steranes and hopanes. Compared with P. aeruginosa XJ16, A. lwoffii XJ19 biodegraded more N1 class and produced more intermediate products (N1O1 and N1O2 classes). The efficiency of biodegradation of O1 varied with the type of microorganism. Intermediate products, such as alkylphenols, alkylnaphthols naphthols, and phenyl phenols, were produced by P. aeruginosa XJ16, whereas A. lwoffii XJ19 biodegraded these products. After biodegradation, the saturated fatty acids in Pa-90 and Al-90 decreased through the monoterminal, diterminal, and subterminal oxidation of n-alkane. However, naphthenic acids with 1-6 rings produced from terminal oxidation of naphthenes or dearomatization of aromatic hydrocarbons increased. Overall, these findings provided key insights into the biodegradation of polar compounds by different bacterial species.
Copyright © 2020 American Chemical Society.

ISSN号：0887-0624

卷、期、页：卷34期5：5553-5565

发表日期：2020-05-21

期刊分区（SCI为中科院分区）：三区

收录情况：EI（工程索引）

发表期刊名称：Energy and Fuels

通讯作者：刘源,Zhu, Yingjia,Fei, Caisheng,Shen, Zidi,Ying, Yuxi

第一作者：万云洋

论文类型：期刊论文

论文概要：刘源,万云洋,Zhu, Yingjia,Fei, Caisheng,Shen, Zidi,Ying, Yuxi，Impact of Biodegradation on Polar Compounds in Crude Oil: Comparative Simulation of Biodegradation from Two Aerobic Bacteria Using Ultrahigh-Resolution Mass Spectrometry，Energy and Fuels，2020，卷34期5：5553-5565

论文题目：Impact of Biodegradation on Polar Compounds in Crude Oil: Comparative Simulation of Biodegradation from Two Aerobic Bacteria Using Ultrahigh-Resolution Mass Spectrometry