摘要：Direct extraction of deoxyribonucleic acid (DNA) from petroleum is the most fundamental and most important step for analyses of its microbial community and diversity. The spincolumn based extraction method (SE) and the magnetisable solid phase extraction method (ME) were used to obtain the total DNA of microbial genome of 12 petroleum samples, respectively. And their difference of microbial diversity and community were analyzed further more through Illumina high throughput sequences. The results show that the DNA mass concentration and purity (A260 / A280 ratio) are 20. 17±31. 83 ng/ μL and 1. 01±0. 63 of SE, and 11. 14±17. 27 ng/ μL and 1. 29±0. 20 of ME, respectively. SE detects 30 phyla and 507 genera, while ME detects 38 phyla and 677 genera, in which the relative content of unclassified and uncultured genera are 1. 12% and 0. 20% (SE), 1. 24% and 1. 10% (ME), respectively. Unique genera are detected by both methods (SE: 65 genera and ME: 235 genera). SE detects reservoir microorganisms such as Geobacter, and ME detects suspected reservoir microorganisms such as Xanthobacter. Both methods have advantages and disadvantages in petroleum DNA extraction. SE has a higher extraction mass concentration and better response to oil reservoir microorganisms. ME has a slightly stronger ability to detect microbial diversity in petroleum, but both methods have strong detection bias at the taxonomic level. ? 2025 University of Petroleum, China. All rights reserved.

ISSN号：1673-5005

卷、期、页：卷49期4：224-230

发表日期：2025-08-01

期刊分区（SCI为中科院分区）：无

收录情况：EI（工程索引）

发表期刊名称：Zhongguo Shiyou Daxue Xuebao (Ziran Kexue Ban)/Journal of China University of Petroleum (Edition of Natural Science)

参与作者：Tao, Jianqiang,Hu, Chunyu,Yang, Yuanliang,Gao, Zhiwei

通讯作者：熊驷骏,樊宝旭,徐朋朋

第一作者：万云洋,赵国屏

论文类型：期刊论文

论文概要：熊驷骏,Tao, Jianqiang,万云洋,Hu, Chunyu,樊宝旭,Yang, Yuanliang,徐朋朋,Gao, Zhiwei,赵国屏，DNA extraction from petroleum byspincolumn based extraction and magnetisable solid phase extraction，Zhongguo Shiyou Daxue Xuebao (Ziran Kexue Ban)/Journal of China University of Petroleum (Edition of Natural Science)，2025，卷49期4：224-230

论文题目：DNA extraction from petroleum byspincolumn based extraction and magnetisable solid phase extraction