摘要：The identification of microorganisms using single-temperatures pyrolysis gas chromatography (ST-PyGC) has limitations, for example, the risk of missing characteristic peaks that are essential to the chemotaxonomic interpretation. In this paper, we proposed a new multi-temperature PyGC (MT-PyGC) method as an alternative to ST-PyGC, without sacrificing its speed and quality. Six bacteria (three Gram-positive and three Gram-negative), one micro-fungus and one archaeon, representing microorganisms from different domains, were analyzed by MT-PyGC. It is found that MT pyrograms cover a more complete range of characteristic peaks than ST. Coupling with thermogravimetric analysis, chemotaxonomic information extracted from pyrograms by MT-PyGC have the potential for the differentiation of microorganisms from environments including deep subterranean reservoirs and biomass conversion/biofuel production.

关键字：microorganism identification; multi-temperatures pyrolysis; thermogravimetric analysis; archaea; bacteria; fungi

卷、期、页：卷: 10期: 12

发表日期：2022-12-01

影响因子：4.128000

期刊分区（SCI为中科院分区）：二区

收录情况：SCIE（科学引文索引网络版）

发表期刊名称：MICROORGANISMS

通讯作者：朱迎佳,江亮,Luo, Na

第一作者：万云洋

论文类型：期刊论文

论文概要：万云洋,朱迎佳,江亮,Luo, Na，Multi-Temperatures Pyrolysis Gas Chromatography: A Rapid Method to Differentiate Microorganisms，MICROORGANISMS，2022，卷: 10期: 12

论文题目：Multi-Temperatures Pyrolysis Gas Chromatography: A Rapid Method to Differentiate Microorganisms